c reactive protein  (R&D Systems)

Journal: Brain, Behavior, & Immunity - Health

Article Title: Inflammation, mental health, and alcohol behaviors: Testing links leveraging a familial community sample

doi: 10.1016/j.bbih.2026.101229

Figure Lengend Snippet: – LME results for the MDD outcome across pro-inflammatory (Pro-Inf) and CRP main effects . MDD = Major depressive disorder; Fruit/Veg = Fruit/Vegetable intake; DepMed = Depression medication use; Pro-Inf = pro-inflammatory; CRP = C-reactive protein; B = regression weight; SE = standard error. Blue = positive value; Orange = negative value; ∗ = p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Each plasma sample was assayed in triplicate using a multiplex of pro- and anti-inflammatory cytokines (Quanterix Human Cytokine 10 Plex Array) or C-reactive protein (CRP; R&D Systems Quantikine QuicKit ELISA).

Techniques:

Journal: Brain, Behavior, & Immunity - Health

Article Title: Inflammation, mental health, and alcohol behaviors: Testing links leveraging a familial community sample

doi: 10.1016/j.bbih.2026.101229

Figure Lengend Snippet: LME results for the pro-inflammatory and CRP outcomes and alcohol use main effects. AlcQ = Alcohol quantity; AlcF = Alcohol frequency; F∗Q = Alcohol frequency∗alcohol quantity; AUD = Alcohol use disorder; Fruit/Veg = Fruit/Vegetable intake; DepMed = Depression medication use; Pro-Inf = pro-inflammatory; CRP = C-reactive protein; B = regression weight; SE = standard error. Blue = positive value; Orange = negative value; ∗ = p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Each plasma sample was assayed in triplicate using a multiplex of pro- and anti-inflammatory cytokines (Quanterix Human Cytokine 10 Plex Array) or C-reactive protein (CRP; R&D Systems Quantikine QuicKit ELISA).

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Cordycepin Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Inhibiting IL-6/IL-6R-Mediated p38 MAPK and NF-κB Activation Through Adenosine A 2A Receptor Stimulation

doi: 10.2147/DDDT.S575035

Figure Lengend Snippet: Cordycepin mitigates 2% DSS-induced damage in NCM460 cells. ( A ) Effects of different concentrations of DSS on the viability of NCM460 cells. ( B ) Effects of different concentrations of cordycepin (COR) on the viability of NCM460 cells. NCM460 cells were treated with 2% DSS and / or 1 μmol/L (COR-1), 10 μmol/L (COR-10) COR for 24 h, the release levels of ( C ) LDH, ( D ) IL-1β, ( E ) IL-6, and ( F ) TNF-α in the supernatant were measured by using Elisa. ( G ) Representative images of PI staining and the percentage of PI-positive cells, scar bar = 100 μm. ( H ) The protein expression of ZO-1 in NCM460 cells was determined by using Western blotting assay. The results were representative of three independent experiments and expressed as mean ± SEM. Data were compared using two-tailed Student’s t tests. # P < 0.05, ## P < 0.01, ### P < 0.001 and #### P < 0.0001 vs. control group; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 vs. the 2% DSS group.

Article Snippet: Serum CRP levels were measured using a mouse-specific sandwich ELISA kit (E-EL-M0053, Elabscience).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Two Tailed Test, Control

Journal: Drug Design, Development and Therapy

Article Title: Cordycepin Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Inhibiting IL-6/IL-6R-Mediated p38 MAPK and NF-κB Activation Through Adenosine A 2A Receptor Stimulation

doi: 10.2147/DDDT.S575035

Figure Lengend Snippet: Cordycepin abates DSS-induced damage in NCM460 cells by activating adenosine receptor A 2A . ( A ) The cAMP levels in NCM460 cells under treatment with 2% DSS and/or 1 μmol/L and 10 μmol/L COR. ( B ) The cAMP levels in NCM460 cells under treatment with 2% DSS, 10 μmol/L COR, 5 μmol/L SCH58261 (SCH), and / or 5 μmol/L DPCPX for 24 h. The release levels of ( C ) LDH, ( D ) IL-1β, ( E ) IL-6, and ( F ) TNF-α were measured by using Elisa. ( G ) Representative images of PI staining and the percentage of PI-positive cells, scar bar = 100 μm. ( H – J ) The protein expression of ZO-1, A 2A AR and A 1 AR in NCM460 cells was determined by using Western blotting assay. The results were representative of three independent experiments and expressed as mean ± SEM. Data were compared using two-tailed Student’s t tests. vs. control group; # P < 0.05, vs. control group; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the 2% DSS group.

Article Snippet: Serum CRP levels were measured using a mouse-specific sandwich ELISA kit (E-EL-M0053, Elabscience).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Two Tailed Test, Control

Journal: Drug Design, Development and Therapy

Article Title: Cordycepin Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Inhibiting IL-6/IL-6R-Mediated p38 MAPK and NF-κB Activation Through Adenosine A 2A Receptor Stimulation

doi: 10.2147/DDDT.S575035

Figure Lengend Snippet: SCH58261 blocks cordycepin’s amelioration of intestinal inflammation and gut barrier function in DSS-induced colitis mice. ( A – C ) Level of colonic IL-1β, IL-6, and TNF-α among the Control, DSS, DSS+COR-L and DSS+COR-H group were detected by using Elisa. ( D ) Representative images of AB-PAS staining of colonic tissue. The arrow indicates the goblet cells. ( E ) Number of goblet cells in colonic tissue. ( F ) Representative images of TUNEL staining in colonic tissue, scale bar = 50 μm. ( G ) Percentage of TUNEL-positive cells (%). ( H ) The protein expression of ZO-1 and Occludin in colons was determined by using Western blotting assay. ( I ) and ( J ) Serum level of D-lactate and diamine oxidase (DAO) were measured by using Elisa. ( K ) The serum cAMP levels among the control, DSS, DSS+COR-L, DSS+COR-H group and DSS+SCH+COR-H group. Data were presented as the means ± SEM of six-eight mice in each group and were compared using two-tailed Student’s t tests. # P < 0.05, ## P < 0.01, ### P < 0.001 and #### P < 0.0001 vs. control group; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 vs. the DSS model (DSS) group.

Article Snippet: Serum CRP levels were measured using a mouse-specific sandwich ELISA kit (E-EL-M0053, Elabscience).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Staining, TUNEL Assay, Expressing, Western Blot, Two Tailed Test